Creative BioMart, a biotech company that has always been focusing on developing high quality protein products and efficient protein manufacturing techniques, provides services on enzymatic assays to assist scientists focused on enzyme and protein research.
The vast majority of catalytic biological processes are accomplished by enzymes that provide multiple functions in living organisms. They are essential for metabolic processes, cell signaling and regulation, and can produce movement and active transport of molecules. Enzymatic reactions can specifically convert substrate molecules into chemically modified products. These modifications include proteolytic or glycolytic degradation, chemical modifications such as phosphorylation, acylation, glycosylation or oxidative and reductive processes. Dysregulation of enzyme activity can lead to disease states. As a result, enzymes are considered attractive targets for pharmacological intervention in drug therapy.
Enzyme activity assay at Creative BioMart first involves immunocapture of the target protein and then measures substrate or product turnover. Separating the enzyme from other proteins that use or produce the same substrate or screened product allows for simple assays, such as ATP, NADH, pyruvate, etc., that undergo a variety of reactions in vivo. “For most of the enzymes for which we had an activity assay kit, we also provided a number of sandwich ELISA assays.” Commented Linna, the chief marketing staff at Creative BioMart. By combining the two, specific activity data can be generated. In addition, since the enzyme is separated from other cellular components, it is possible, for example, to determine the posttranslational modifications (PTMs) it contains at the time of the assay by subsequent mass spectrometry analysis or by reaction with PTM-specific antibodies.
Over the years, Creative BioMart has accumulated abundant experience in the design and manufacture of active enzymes and their substrates. Now it is able to support the development of an ever-expanding portfolio of biochemical assays. Services on enzymatic assays at Creative BioMart mainly include:
Kinetic evaluation of enzyme activity and inhibition
Inhibitor screening and validation
IC50 determination of drug compounds
Enzyme-inhibitor studies analyzing mode of action
Investigations on reversibility of enzyme inhibition
Substrate profiling analyzing selectivity of enzymes
To get a whole view of the enzyme activity assay provided by Creative BioMart, you can visit https://www.creativebiomart.net/Enzyme-Activity-Assay.htm.
About Creative BioMart
Starting from a small supplier of proteins and enzymes for academic institutes and biotech companies, Creative BioMart has always been focusing on developing high quality protein products and efficient protein manufacturing techniques. Over the past decade, our products and services are proved to have served our customers well and our brand has become one of the most trustworthy in the market.