Long oligo synthesis has great importance in today’s biological research. However, the long oligo synthesis can be challenging. The longer the oligonucleotide, the more difficult it can be to isolate a pure, biologically active product. Even after many tries, no chemical reaction has been successful to accomplish 100% efficiency.

In an oligonucleotide synthesis, researchers have to perform four chemical reactions coupling, capping, oxidation and deblocking. And, any mistake and flaw in any of these reactions, will result in the generation of failure sequences. When the number of failure sequences increases, it can be virtually impossible to acquire pure oligos. All these lead to a mixture of n-1 deletion mutations that are possible to be potentially disastrous in biological applications.

The researchers need to make sure that it must be stressed that the reagents used for long oligo synthesis should be fresh and of the highest purity. Also, they need to make sure that the diluent and activator especially should be dry and fresh. Inferior reagents will result in deletion mutations, while potentially insertion mutations and chain branching. In case the long oligo synthesis is carried out efficiently, the level of purification researchers desire can be accomplished.

Importance of Oligonucleotide Synthesis
Oligonucleotide synthesis is basically the chemical synthesis of relatively short fragments of nucleic acids with a defined chemical structure. This particular technique is highly useful in current laboratory practice as it ensures rapid and low-cost access to custom-made oligonucleotides of the desired sequence for various applications.

Uses of Oligonucleotide Synthesis
The applications involve using molecular beacons to track the PCR amplification process, nuclease footprinting to check the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

If you need long oligo synthesis or oligonucleotide synthesis services, reach out to a top biology research support company that offers modified oligonucleotides with a wide range of modifications such as Linkers, Spacers, Quenchers, Fluorophores, Modified Bases, Phosphorylation, Probes and more. To learn further, contact the service providers and get the desired products for your research.

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