In 1977, English biochemist Frederick Sanger and his colleagues developed Sanger sequencing, often known as the "chain termination method." This approach is used to determine the nucleotide base sequence in a fragment of DNA. The "gold standard" for validating DNA sequences, including those already sequenced using next-generation sequencing, is Sanger sequencing, which has a base accuracy of 99.99 percent (NGS). The Human Genome Project employed Sanger sequencing to identify the sequences of relatively tiny segments of human DNA (900 bp or less). Larger DNA segments and, finally, whole chromosomes were assembled using these pieces.
Nowadays, many online portals offer Sanger sequencing at a very competitive rate.
How Does Sanger Sequencing Work?
A DNA primer complementary to the template DNA is used as a starting point for DNA synthesis in Sanger sequencing. The polymerase extends the primer by adding the corresponding dNTP to the template DNA strand in the presence of the four deoxynucleotide triphosphates (dNTPs: A, G, C, and T). Four dideoxynucleotide triphosphates (ddNTPs: ddATP, ddGTP, ddCTP, and ddTTP) tagged with a unique fluorescent dye are employed to terminate the synthesis reaction to determine which nucleotide is incorporated into the chain of nucleotides. Following synthesis, the reaction products are placed into four lanes of a single gel and subjected to gel electrophoresis, depending on the different chain-terminating nucleotides. The sequence of DNA is thus determined based on its diameters.
How to get Sanger Sequencing?
Bio Basic offers sanger sequencing online at competitive rates. We offer Sanger DNA sequencing, Plasmid purification, and PCR cleanup.
You will get free shipping on orders over 100 samples. On their website, you can also check the pricing and turnaround options.