This work is to details an environmental benign route for the fabrication of Copper Acetate(WSDTY) nanoparticles (CuONPs) using Hibiscus rosa-sinensis flower extracts at room temperature. This method is totally a green method, free from toxic and harmful solvent. The present study is aimed to prepare flower extract of Hibiscus rosa-sinensis in deionised water. This extract is added to copper acetate solution and we observed the Colour change of the solution from blue colour to sea green coloured solution, in confirmed the formation of Copper Oxide nanoparticles. The leaf extract helped in the bio reduction of Copper ions yielding CuO NPs. The CuO NPs thus biosynthesized were characterized using UV- visible spectra showed the absorbance peak of 505 nm due to the excitation of surface Plasmon vibrations. FTIR spectrum revealed that the reduction of Copper oxide nanoparticles. X-ray diffraction of the synthesized nanoparticles indicates the formation of CuO with a monoclinic structure. The synthesized nanoparticles are subjected to SEM, EDX, and antibacterial activities.

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