Recently, BOC Sciences, relying on its advanced fluorescence technology platform, has launched a variety of super-sensitive fluorescent probes for the detection of reactive oxygen species.
Both the oxygen in the air and the dissolved oxygen in the water have high oxidizing properties. All parts of the human body in the air are constantly being corroded by oxygen. In other words, oxygen will damage our body at the cellular level. This damage is mainly carried out by the reactive oxygen species (ROS) in our body. Reactive oxygen species (ROS) include superoxide free radicals, hydrogen peroxide and its downstream products peroxides and hydroxylates, etc., and participate in the growth, proliferation, development, differentiation, aging and apoptosis of cells, as well as many physiological and pathological processes. Fluorescent probes for measuring ROS play a key role in studying the biological effects of ROS.
BOC Sciences has launched a series of high-resolution, high-sensitivity fluorescent probes to study various reactive oxygen species.
Dihydroethidium (also called hydroethidium) is a fluorescent probe used to detect superoxide anions in cells. Dihydroethidium can be oxidized by superoxide anions in the cell to generate ethidium bromide, which is a fluorescent product. Ethidium bromide can accumulate in cells to bind to RNA or DNA, thereby increasing fluorescence intensity and producing red fluorescence.
Dihydrorhodamine 123 (DHR)
Dihydrorhodamine 123 is a cell-permeable fluorescent probe that is often used to detect hydrogen peroxide in cells. Dihydrorhodamine reacts with ROS to produce fluorescent rhodamine 123. Rhodamine 123 is a positively charged fat-soluble molecule that can be used as a fluorescent mitochondrial dye. In addition, high concentrations of dihydrorhodamine can detect the production of singlet oxygen in the mitochondria.
H2DCFDA is a non-fluorescent, fat-soluble substance with cell membrane permeability. It is hydrolyzed by esterase into water-soluble 2′,7′-dichlorodihydrofluorescein (DCFH) in the cell. DCFH then reacts with ROS to produce 2′,7′-dichlorofluorescein (DCF) with strong green fluorescence. H2DCFDA is suitable for detecting reactive oxygen species and nitric oxide in cells and determining the total oxidative stress level.
There are corresponding probes for studying different ROS. To obtain reliable test results, it is necessary to select an appropriate probe, comprehensively consider various influencing factors, and reasonably analyze the data.
For more information about fluorescent probes for ROS detection, please visit the website: https://probes.bocsci.com/nitric-oxide-no-reactive-oxygen-species-ros.html.
About BOC Sciences
With many years of experience in the management of fluorescent probes, BOC Sciences is familiar with and understands the market of fluorescent probes, and provides a comprehensive range of fluorescent probes and dyes for scientists and researchers in more than 160 countries/regions. In the past two decades, the company has relied on professional sales and technical service team, an independent fluorescent technology platform, and the principle of “quality assurance” to efficiently meet the needs of various customers.